Griet Glorieux lab


Prof. Dr. Griet Glorieux

Research topic

Chronic kidney disease (CKD) is characterised by the accumulation of a myriad of uraemic retention compounds and a disruption of the intestinal barrier function contributing to chronic micro-inflammation and an increased risk for cardiovascular disease (CVD), the major cause of death in CKD patients. The Nephrology Research Unit at the Clinical Nephrology Unit of the Ghent University Hospital has a long term experience in uraemic toxicity. Many pathophysiological effects of uraemic retention compounds on different cell types (renal and cardio-vascular) have been demonstrated over the past decade, hence the name "uraemic toxins" (www.uremic-toxins.org). Many of these compounds indicated as most toxic by in vitro, in vivo and by clinical association (phenolic and indolic compounds) are generated in the colon and are very difficult to remove by dialysis therapy. For early stage prevention of inflammation and CVD in CKD and in addition to dialysis therapy in end stage kidney disease, an important alternative strategy could thus be to target the gut microbiota to decrease the primary generation of these toxins and to decrease chronic micro-inflammation as a consequence of a leaky gut. The ultimate goal is to develop therapeutic measures/therapeutic strategies to alter the intestinal generation of uraemic toxins, resulting in lowering their circulating levels, aiming at decreasing inflammation and cardiovascular morbidity and mortality in CKD patients.

Areas of expertise

  • Biobanking of biospecimens of CKD patients, including serum, plasma, urine, buffy-coat for DNA extraction (n=~600 with 2 year sample follow-up and 5 year outcome follow-up) and stool samples [CKD n=108 (cross-sectional); HD n=144 (longitudinal); PD (being collected (longitudinal)]
  • In vitro and in vivo (rat) models for evaluating uremic toxicity (leukocyte activation and leukocyte-endothelial cross-talk)
  • Quantification of intestinally generated uraemic toxins by UPLC

Selected publications

  1. Gryp T et al. p-Cresyl sulfate.
    Toxins (basel) 2017 Jan 29; 9(2).
  2. Vanholder and Glorieux. The Intestine and the Kidneys: a Bad Marriage Can Be Hazardous.
    Clin. Kidney J. 2015, 8, 168-179.
  3. Schepers et al. Transcriptome Analysis in Patients With Chronic Kidney Disease on Hemodialysis Disclosing a Key Role for CD16+CX3CR1+ Monocytes.
    PLoS One 2015, 10, e0121750.
  4. Neirynck et al. Inflammatory Cytokines and Leukocyte Oxidative Burst in Chronic Kidney Disease: Culprits or Innocent Bystanders?
    Nephrol. Dial. Transplant. 2015, 30, 943-951.
  5. Vanholder et al. The Uremic Toxicity of Indoxyl Sulfate and P-Cresyl Sulfate: a Systematic Review.
    J. Am. Soc. Nephrol. 2014, 25, 1897-1907.
  6. Pletinck et al. Protein-bound uremic toxins stimulate crosstalk between leukocytes and vessel wall.
    J. Am. Soc. Nephrol. 2013; 24: 1981-1994.

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